For research collaborators
FRAGSIGN, powered by SDF-Index, supports laboratory research into sperm DNA fragmentation. This overview helps researchers and clinician-scientists understand the workflow and its analytical outputs.
Research measurements
DFI and HDS describe different features of the acquired fluorescence data under the assay conditions. Interpretation begins with the protocol, specimen preparation, acquisition quality, and analysis settings.
A consistent report structure supports comparison and review within a research project. It does not by itself establish the validity of a scientific conclusion.
Understanding the output
The proportion of measured sperm events exhibiting increased susceptibility consistent with fragmented DNA under the assay conditions.
DFI is reported as a percentage. Research category assignment should be reviewed with its defined boundaries and method context.
The proportion of measured sperm events exhibiting high DNA stainability under the assay conditions.
HDS is reported separately from DFI and should remain a distinct parameter in research analysis.
Study review
Research teams should consider repeatability, acquisition conditions, quality controls, and the relationship between technical replicates and independent specimens.
Research reporting
Intended use
FRAGSIGN and SDF-Index are For Research Use Only. Not for use in diagnostic procedures. Their outputs are not offered as a basis for patient diagnosis, treatment selection, or individual outcome prediction.
Varen can help your laboratory team explore reagents, training, and analytical requirements.
